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biorad everyblot blocking buffer  (Bio-Rad)


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    Bio-Rad biorad everyblot blocking buffer
    Biorad Everyblot Blocking Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/everyblot+blocking+buffer+biorad/dd-Check+STEC+Droplet+Generation+Oil/pm41309594-313-31-35
    Average 96 stars, based on 268 article reviews
    biorad everyblot blocking buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    Staining:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    Blocking Assay:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..


    Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-tag mouse monoclonal antibody Cell Signaling Technology Cat#2367S, RRID: AB_10691311 Anti-actin C4 monoclonal antibody Sigma Aldrich Cat#MAB1501, RRID: AB_2223041 HRP-conjugated goat anti-mouse antibody Cell Signaling Technology Cat#96714S Bacterial and virus strains E. coli OP50 CGC OP50 E. coli DH5a competent cells Fisher Scientific Cat#18-265-017 E. coli HT115 CGC HT115 Chemicals, peptides, and recombinant proteins Ethyl Methane Sulfonate Sigma Cat#M0880 Cycloheximide Sigma Aldrich Cat#239763 Histamine Sigma Aldrich Cat#H7250 Sodium azide Sigma Aldrich Cat#S-2002 Taq DNA Polymerase Sibgene Cat#SG-1-500 ExTaq DNA polymerase Takara Cat#RR01AM Trioxalen Sigma Aldrich Cat#T6137 FITC Molecular Probes Cat#F143 XmaI New England Biolabs Cat#R0180S NheI-HF New England Biolabs Cat#R3131S HindIII-HF New England Biolabs Cat#R3104L AgeI-HF New England Biolabs Cat#R3552S EcoRI-HF New England Biolabs Cat#R3101L SphI New England Biolabs Cat#R3182L SpeI New England Biolabs Cat#R3133L BamHI New England Biolabs Cat#R3136L PstI New England Biolabs Cat#R0140L NotI New England Biolabs Cat#R0189L XbaI New England Biolabs Cat#R0145L KpnI-HF New England Biolabs Cat#R3142S TRIzol Invitrogen Cat#15-596-026 Bortezomib LC Laboratories Cat#B1408 2x Laemmli Buffer Biorad Cat#1610737 EveryBlot Blocking Buffer Biorad Cat12010020 Chloroform Fisher Scientific Cat#298-500 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat#67-68-5 Tetramisol hydrochloride Sigma Aldrich Cat#L9756 2-mercaptoethanol Sigma Aldrich Cat#M6250 Critical commercial assays RNeasy Mini Elute Cleanup Kit Qiagen Cat#74204 QuantiTect Rev. .. Transcription Kit Qiagen Cat#205311 Gibson Assembly Master Mix New England Biolabs Cat#E2611S QIAprep Spin Miniprep Kit Qiagen Cat#27106 Deposited Data Western Blot Raw Images https://doi.org/10.17632/6k4bfygcs2.1 (Continued on next page) e1 Developmental Cell 59, 1–21.e1–e7, July 8, 2024

    Western Blot:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    RNA sequencing:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    Derivative Assay:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    Software:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..


    Expressing:

    Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-AGO2 Sino Biological Cat#50683-R036; RRID: AB_2860516 anti-AQR (IBP160) Bethyl Laboratories Cat#A302-547A; RRID: AB_1998964 anti-BCLAF1 (BTF) Bethyl Laboratories Cat#A300-608A; RRID: AB_513581 anti-BUD13 Bethyl Laboratories Cat#A303-320A; RRID: AB_10952849 anti-DDX3X Bethyl Laboratories Cat#A300-474A; RRID: AB_451009 anti-DDX6 Abcam Cat# ab174277; RRID: AB_2928958 anti-DGCR8 Abcam Cat#ab191875; RRID: AB_2892625 anti-DROSHA (D28B1) Cell Signaling Technology Cat#3364; RRID: AB_2238644 anti-EFTUD2 Bethyl Laboratories Cat#A300-957A; RRID: AB_805780 anti-FTO Abcam Cat#ab126605; RRID: AB_11127120 anti-GRWD1 Bethyl Laboratories Cat#A301-576A; RRID: AB_1040030 anti-ILF3 Abcam Cat#ab92355; RRID: AB_2049804 anti-LIN28B Bethyl Laboratories Cat#A303-588A; RRID: AB_11125329 anti-NCBP2 Bethyl Laboratories Cat#A302-553A; RRID: AB_2034872 anti-PPIG (SRCyp) Bethyl Laboratories Cat#A302-075A; RRID: AB_1604291 anti-PRPF8 Bethyl Laboratories Cat#A303-921A; RRID: AB_2620270 anti-QKI Abcam Cat#ab126742; RRID: AB_11129508 anti-RPS3 Bethyl Laboratories Cat#A303-841A; RRID: AB_2620192 anti-SF3A3 Bethyl Laboratories Cat#A302-506A; RRID: AB_1966116 anti-SF3B4 Bethyl Laboratories Cat#A303-950A; RRID: AB_2620299 anti-SSB Abcam Cat#ab75927; RRID: AB_10865926 anti-TBRG4 Bethyl Laboratories Cat#A301-392A; RRID: AB_938027 anti-TRA2A Bethyl Laboratories Cat#A303-779A; RRID: AB_11218175 anti-TROVE2 Abcam Cat#ab207416; RRID: AB_2928959 anti-XPO5 Bethyl Laboratories Cat#A303-991A; RRID: AB_2620340 anti-YBX3 (ZONAB) Bethyl Laboratories Cat#A303-070A; RRID: AB_10893576 anti-ZNF622 (ZPR9) Bethyl Laboratories Cat#A304-075A; RRID: AB_2621324 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab205718; RRID: AB_2819160 Goat Anti-Mouse IgG Antibody, HRP conjugate Sigma-Aldrich Cat#12–349; RRID: AB_390192 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins TRI Reagent Sigma-Aldrich Cat#93289 Animal Component-Free Cell Attachment Substrate STEMCELL Technology Cat#07130 MesenCultTM-ACF Plus 500X Supplement STEMCELL Technology Cat#05447 Pro293TM a Serum-free Medium Lonza Cat#BEBP12-764Q GlutaMAXTM Supplement GIBCO Cat#35050061 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 Accutase GIBCO Cat#A11105 PBS, pH 7.4 GIBCO Cat#10010049 CDM-HD Chemically Defined Medium FiberCell Systems Cat#LC5800 DMEM, high glucose, GlutaMAXTM Supplement, pyruvate GIBCO Cat#31966021 (Continued on next page) e1 Cell Genomics 3, 100303, May 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM Corning Cat#10-017-CV Fetal Bovine Serum GIBCO Cat#10270106 Fibronectin bovine plasma Sigma-Aldrich Cat#F4759 MEM Non-Essential Amino Acids Solution (100X) GIBCO Cat#11140050 Gelatine Solution MilliporeSigma Cat#G1393 Critical commercial assays 4–15% Mini-PROTEANTM TGX Stain-FreeTM Protein Gels, 15 well, 15 ml Biorad Cat#4568086 Trans-Blot Turbo Mini 0.2 mm PVDF Transfer Packs Biorad Cat#1704156 Precision Plus ProteinTM Dual Color Standard Biorad Cat#1610394 EveryBlot Blocking Buffer Biorad Cat12010020 Clarity Western ECL Substrate Biorad Cat#1705060 Centricon Plus-70 Centrifugal Filter, 30kDA cutoff Millipore Cat#UFC703008 Aurum Affi-Gel Blue Mini Columns Biorad Cat#7326708 Amicon Ultra-15 Centrifugal Filters Millipore Cat#UFC9030 Micro BCATM Protein Assay Kit ThermoFisher Cat#23235 NuPAGETM LDS Sample Buffer (4X) ThermoFisher Cat#NP0007 Chemi Blot Blocking Buffer Azure Biosystems Cat#AC2148 Radiance Plus Azure Biosystems Cat#AC2103 Deposited data Analyzed small RNAseq data in multiple exRNA atlas studies Murillo et al. 20192 www.exrna-atlas.org Intersected ENCODE eCLIP regions Van Nostrand et al. 202013 https://www.encodeproject.org/ encore-matrix/?type=Experiment&status= released&internal_tags=ENCORE exRNA/RBP intersect data This manuscript - data is publicly available at www.exrna-atlas.org Accessions listed in Table S3 RBC EV sequences This manuscript - data is publicly available at www.exrna-atlas.org EXR-IGHIR1BloodCellEVs-AN Experimental models: Cell lines 293T Flp-in T-REX (female) Thermo Fisher Scientific Cat#R78007 DiFi cell line (female) Laboratory of Robert Coffey Olive et al., 199329 Human Bone Marrow Stromal Cells Derived in ACF Medium (female) STEMCELL Technology Cat#70071 Software and algorithms Samtools 1.3.1 Li et al., 200930 http://www.htslib.org/ BEDTools 2.17 Quinlan and Hall, 201031 https://bedtools.readthedocs.io/en/latest/# R v3.5, v4.0 R Core Team, 202032 https://www.r-project.org/ Image Lab 6.1 Bio-Rad Laboratories https://www.bio-rad.com/en-ch/product/ image-lab-software?ID=KRE6P5E8Z exRBP fingerprinting pipeline This manuscript https://doi.org/10.5281/zenodo.7706896 Stand alone sample intersection tool This manuscript https://doi.org/10.5281/zenodo.7706896 Extracellular RNA Atlas Subramanian et al., 201533 https://exrna-atlas.org Expression Deconvolution (XDec) Murillo et al., 20192 https://github.com/BRL-BCM/XDec Cell Genomics 3, 100303, May 10, 2023 e2 Resource ll OPEN ACCESS Resource ll OPEN ACCESS ..

    other:

    Article Title: Eomes directs the formation of spatially and functionally diverse extraembryonic hematovascular tissues.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Pecam/CD 31 rat IgG antibody BD Pharmingen Cat# 550274; RRID: AB 393571 RFP rabbit IgG Rockland antibodies Cat# 600 401 379 Alexa Fluor 594 donkey anti rabbit IgG Invitrogen/Life Technology Cat# A21207; RRID: AB 141637 Alexa Fluor 488 donkey anti rat IgG Invitrogen/Life Technology Cat# A21208; RRID: AB 2535794 polyclonal HRP conjugated Anti goat IgG abcam Cat# ab6741; RRID: AB 955424 polyclonal HRP conjugated Anti rat IgG Cell Signaling Cat# 7077S; RRID: AB 10694715 Anti T goat polyclonal Santa Cruz Cat# sc 17745; RRID: AB 2200243 Anti EOMES rat monoclonal Thermo Fisher Cat# 14 4875 82; RRID: AB 11042577 Alexa FluorTM 488 anti EOMES rat monoclonal Invitrogen Cat# 53 4875 82; RRID: AB 10854265 Pe/Cy7 anti mouse FLK 1 BioLegend Cat# 136414; RRID: AB 2561606 BV605 rat anti mouse PDGFRA BD Biosciences Cat# 740380; RRID: AB 2740111 APC anti mouse DLK1 R&D Systems Cat# FAB8634A; RRID: AB 2890004 Pe/Cy7 anti mouse CDH5 Thermo Fisher Scientific Cat# 25 1441 82; RRID: AB 2573402 APC anti mouse CD41 Thermo Fisher Scientific Cat# 17 0411 82; RRID: AB 1603237 Chemicals, peptides, and recombinant proteins Vectashield mounting medium with DAPI Vector Laboratories Cat# H 1200 DAPI BD Bioscience Cat# 564907 eBioscienceTM Fixable Viability Dye eFluorTM 450 Invitrogen Cat# 65 0863 14 PD0325901 Synthesized by the MRC Protein Phosphorylation Unit, Division of Signal Transduction Therapy at the University of Dundee, UK N/A CHIR99021 Synthesized by the MRC Protein Phosphorylation Unit, Division of Signal Transduction Therapy at the University of Dundee, UK N/A LIF Millipore Cat# ESG1107 Recombinant human VEGF R&D Systems Cat# 293 VE Recombinant human BMP4 R&D Systems Cat# 314 BP Recombinant human/mouse/rat Activin A R&D Systems Cat# 338 AC dTAG 13 Tocris / Bio Techne Cat# 6605 β mercaptoethanol Sigma Aldrich Standard reagent TrypLE Express Thermo Fisher Scientific Cat# 12605010 Neurobasal media Gibco Cat# 21103049 DMEM/F12 Gibco Cat# 11320033 B27 Gibco Cat# 17504044 BSA Gibco Cat# 15260037 Pen/Strep Thermo Fisher Scientific Cat# 15140122 L glutamine Thermo Fisher Scientific Cat# 25030024 DMSO Merck D2650 100ML Critical commercial assays DCTM Protein Assay Kit I Biorad Cat# 5000111 Laemmli buffer Biorad Cat# 1610747 EveryBlot blocking buffer Biorad Cat# 12010020 ECLTM prime Western Blotting Detection Reagent Sigma Aldrich Cat# GERPN2236 (Continued on next page) Developmental Cell 60, 1–12.e1–e9, December 15, 2025 e1

    Magnetic Cell Separation:


    Magnetic Beads:


    Reverse Transcription:


    SYBR Green Assay:


    Recombinant:


    Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-tag mouse monoclonal antibody Cell Signaling Technology Cat#2367S, RRID: AB_10691311 Anti-actin C4 monoclonal antibody Sigma Aldrich Cat#MAB1501, RRID: AB_2223041 HRP-conjugated goat anti-mouse antibody Cell Signaling Technology Cat#96714S Bacterial and virus strains E. coli OP50 CGC OP50 E. coli DH5a competent cells Fisher Scientific Cat#18-265-017 E. coli HT115 CGC HT115 Chemicals, peptides, and recombinant proteins Ethyl Methane Sulfonate Sigma Cat#M0880 Cycloheximide Sigma Aldrich Cat#239763 Histamine Sigma Aldrich Cat#H7250 Sodium azide Sigma Aldrich Cat#S-2002 Taq DNA Polymerase Sibgene Cat#SG-1-500 ExTaq DNA polymerase Takara Cat#RR01AM Trioxalen Sigma Aldrich Cat#T6137 FITC Molecular Probes Cat#F143 XmaI New England Biolabs Cat#R0180S NheI-HF New England Biolabs Cat#R3131S HindIII-HF New England Biolabs Cat#R3104L AgeI-HF New England Biolabs Cat#R3552S EcoRI-HF New England Biolabs Cat#R3101L SphI New England Biolabs Cat#R3182L SpeI New England Biolabs Cat#R3133L BamHI New England Biolabs Cat#R3136L PstI New England Biolabs Cat#R0140L NotI New England Biolabs Cat#R0189L XbaI New England Biolabs Cat#R0145L KpnI-HF New England Biolabs Cat#R3142S TRIzol Invitrogen Cat#15-596-026 Bortezomib LC Laboratories Cat#B1408 2x Laemmli Buffer Biorad Cat#1610737 EveryBlot Blocking Buffer Biorad Cat12010020 Chloroform Fisher Scientific Cat#298-500 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat#67-68-5 Tetramisol hydrochloride Sigma Aldrich Cat#L9756 2-mercaptoethanol Sigma Aldrich Cat#M6250 Critical commercial assays RNeasy Mini Elute Cleanup Kit Qiagen Cat#74204 QuantiTect Rev. .. Transcription Kit Qiagen Cat#205311 Gibson Assembly Master Mix New England Biolabs Cat#E2611S QIAprep Spin Miniprep Kit Qiagen Cat#27106 Deposited Data Western Blot Raw Images https://doi.org/10.17632/6k4bfygcs2.1 (Continued on next page) e1 Developmental Cell 59, 1–21.e1–e7, July 8, 2024

    Virus:

    Article Title: Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-tag mouse monoclonal antibody Cell Signaling Technology Cat#2367S, RRID: AB_10691311 Anti-actin C4 monoclonal antibody Sigma Aldrich Cat#MAB1501, RRID: AB_2223041 HRP-conjugated goat anti-mouse antibody Cell Signaling Technology Cat#96714S Bacterial and virus strains E. coli OP50 CGC OP50 E. coli DH5a competent cells Fisher Scientific Cat#18-265-017 E. coli HT115 CGC HT115 Chemicals, peptides, and recombinant proteins Ethyl Methane Sulfonate Sigma Cat#M0880 Cycloheximide Sigma Aldrich Cat#239763 Histamine Sigma Aldrich Cat#H7250 Sodium azide Sigma Aldrich Cat#S-2002 Taq DNA Polymerase Sibgene Cat#SG-1-500 ExTaq DNA polymerase Takara Cat#RR01AM Trioxalen Sigma Aldrich Cat#T6137 FITC Molecular Probes Cat#F143 XmaI New England Biolabs Cat#R0180S NheI-HF New England Biolabs Cat#R3131S HindIII-HF New England Biolabs Cat#R3104L AgeI-HF New England Biolabs Cat#R3552S EcoRI-HF New England Biolabs Cat#R3101L SphI New England Biolabs Cat#R3182L SpeI New England Biolabs Cat#R3133L BamHI New England Biolabs Cat#R3136L PstI New England Biolabs Cat#R0140L NotI New England Biolabs Cat#R0189L XbaI New England Biolabs Cat#R0145L KpnI-HF New England Biolabs Cat#R3142S TRIzol Invitrogen Cat#15-596-026 Bortezomib LC Laboratories Cat#B1408 2x Laemmli Buffer Biorad Cat#1610737 EveryBlot Blocking Buffer Biorad Cat12010020 Chloroform Fisher Scientific Cat#298-500 Dimethyl sulfoxide (DMSO) Sigma Aldrich Cat#67-68-5 Tetramisol hydrochloride Sigma Aldrich Cat#L9756 2-mercaptoethanol Sigma Aldrich Cat#M6250 Critical commercial assays RNeasy Mini Elute Cleanup Kit Qiagen Cat#74204 QuantiTect Rev. .. Transcription Kit Qiagen Cat#205311 Gibson Assembly Master Mix New England Biolabs Cat#E2611S QIAprep Spin Miniprep Kit Qiagen Cat#27106 Deposited Data Western Blot Raw Images https://doi.org/10.17632/6k4bfygcs2.1 (Continued on next page) e1 Developmental Cell 59, 1–21.e1–e7, July 8, 2024



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